PHEAR

OK, so never having handled nucleic acids in anger before (shut up at the back), today I am actually starting my reverse-transcription PCR optimisation. Cue much messing about in UV cabinets and miscalculating dilutions of mastermix.

Have already found out that I don't have enough kit to fully optimise the reaction for all of my primer pairs, let alone do the experiment that I bought the kit for in the first place. Replicates, so many replicates... because 5 primer pairs are more likely to throw up a working scenario than one. Biology, such a frikkin fuzzy science.

PHEAR

OK, so never having handled nucleic acids in anger before (shut up at the back), today I am actually starting my reverse-transcription PCR optimisation. Cue much messing about in UV cabinets and miscalculating dilutions of mastermix.

Have already found out that I don't have enough kit to fully optimise the reaction for all of my primer pairs, let alone do the experiment that I bought the kit for in the first place. Replicates, so many replicates... because 5 primer pairs are more likely to throw up a working scenario than one. Biology, such a frikkin fuzzy science.

A Pain In The Asp

...The best laid plans o' mice an 'men
Gang aft agley,
An'lea'e us nought but grief an' pain,
For promis'd joy!...

Apologies, maracacha, but my carefully planned, naturalistic floral lariat that I spent all that time dragging you round Covent Garden to buy beads for turned into a snake when I wasn't looking!



Don't hate the snake!!

A Pain In The Asp

...The best laid plans o' mice an 'men
Gang aft agley,
An'lea'e us nought but grief an' pain,
For promis'd joy!...

Apologies, maracacha, but my carefully planned, naturalistic floral lariat that I spent all that time dragging you round Covent Garden to buy beads for turned into a snake when I wasn't looking!



Don't hate the snake!!