its one of many with the nitrocellulose ;) but yeah one of the ones with antibodies, if you choose to look at yer proteins that way
I've extracted with Tris/SDS (gloopy), sonicated (buzzy), quantified (purple and graphy), denatured and reduced (hot and smelly), electrophoresed (bubbly), blotted (pernickety!) and now I just have to identify my proteins (might involve a lot of serum and x-ray film-y).
the whole thing's taken me about a week so far and I have a feeling that one of my membranes did't saturate properly with transfer buffer, and I've lost the top two rungs of one of my standards.
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but yeah one of the ones with antibodies, if you choose to look at yer proteins that way
I've extracted with Tris/SDS (gloopy), sonicated (buzzy), quantified (purple and graphy), denatured and reduced (hot and smelly), electrophoresed (bubbly), blotted (pernickety!) and now I just have to identify my proteins (might involve a lot of serum and x-ray film-y).
the whole thing's taken me about a week so far and I have a feeling that one of my membranes did't saturate properly with transfer buffer, and I've lost the top two rungs of one of my standards.
ARGH